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Protein ladder in western blot4/9/2024 Quality Control :ĥ μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. Contents :Īpproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25☌), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) glycerol. Apply more for thicker (>1.5 mm) or larger gel.2~3 μl per well for general Western transferring.With a range of fluorescent dyes and antibodies for western blot. Multiplex fluorescent western blotting provides accurate, quantitative results, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot, which makes this technique increasingly popular. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 5 Steps to Multiplexed Fluorescent Western Blotting.Migration patterns of BlueRAY Prestained Protein Ladder in different electrophoresis conditions are listed below : Guide for Molecular Weight Estimation (kDa) The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated by SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
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